Technical skills: • ELISA and CLIA development for Thyroid • Rapid assay development for various lateral flow assay and flow through assay • Colloidal Gold preparation (20nm,40nm and HDE) • Conjugation proteins and antibodies with various types colloidal gold • Conjugation of antibody/antigen with HRP • Monoclonal and polyclonal antibody purification by various chromatography techniques • Native and recombinant antigen purification • Purification of various plasma proteins • Protein characterization by SDS PAGE and western blot • Protein estimation by various methods • Stability studies of various products  Hybridoma Technology • Maintenance of cell line (Mouse Myeloma sp2/0, B and T cell line) • Hybridoma Fusion for raising a new monoclone • Sub cloning and screening of new monoclone • Isotyping and characterization of monoclonal antibody(New monoclonal) • Production of monoclonal antibody both in vivo and in vitro model • Development of new polyclonal antibody in rabbit • Production of polyclonal antibody in vivo model • Animal handling: Mouse (Balb/c, Nude mice), Rabbit • Immunization : Mouse (Balb/C, Nude mice), Rabbit, Goat, Chicken • Phlebotomy of human , mice and rabbit